Fig. 1

SHH protein and mRNA levels are increased in GC tissue. a SHH protein expression was analyzed using western blot in tumor (T) and normal (N) gastric tissues in 4 typical samples. b Scatter plots of the densitometrical data show the distribution of SHH expression in tumor and normal gastric tissues (n = 30). c SHH gene expression was analyzed using qRT-PCR in tumor and normal gastric tissues (n = 30). d Serum SHH was analyzed using ELISA in GC patients (n = 10) and control subjects (n = 10)