Fig. 6

Autocrine SHH promotes cell proliferation through a PLCγ1-dependent pathway in GC cell lines. a Representative western blot demonstrating phosphorylation of PLCγ1 and ERK1/2 in AGS and SGC-7901 cells following Recombination Human SHH (rhSHH) treatment. b Western blot experiments demonstrated that Condition Medium (CM) activated phosphorylation of PLCγ1 and ERK1/2 in AGS and SGC-7901 cells, with or without SHH-neutralizing antibody (SHH-NA) treatment. c GC cell proliferation in response to the PLCγ1 inhibitor (U73122) in AGS cells. d GC cell proliferation in response to the PLCγ1 inhibitor (U73122) in SGC-7901 cells. e After U73122 treatment, protein levels were analyzed using western blot, with GAPDH used as a loading control. Mean ± SEM, t-test, *P < 0.05, **P < 0.01,***P < 0.001