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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: EphA1 activation promotes the homing of endothelial progenitor cells to hepatocellular carcinoma for tumor neovascularization through the SDF-1/CXCR4 signaling pathway

Fig. 4

The effect of EphA1 on HCC angiogenesis is SDF-1 dependent in vitro. a WB of SDF-1 in ephrinA1-Fc-activated HLE cells transfected with SDF-1 scrRNA or SDF-1 siRNA, with β-actin as a control for protein loading. b The SDF-1 protein concentration in conditioned medium from ephrinA1-Fc-activated HLE cells transfected with SDF-1 scrRNA or SDF-1 siRNA, evaluated with ELISA. Asterisks indicate significant differences (**P < 0.01), and “ns” indicates an insignificant difference. c The abilities of EPCs to incorporate into the HUVECs (C1), migrate (C2), chemotax to HLE cells (C3), and form tubular structures (C4) were measured after treatment with conditioned medium from ephrinA1-Fc-activated HLE cells transfected with SDF-1 scrRNA or SDF-1 siRNA. The data represent the mean ± SD of three independent experiments. Asterisks indicate significant differences (**P < 0.01; ***P < 0.001)

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