Fig. 3
B55δ knockdown partially reverses the sensitivity of HepG2 cells to cDDP. HepG2-shGFP and HepG2-sh2R2D cells were treated with or without 2.5 μg/ml cDDP for 12 h. a Cell cycle distribution was analyzed by FCM. b Cell migratory ability was detected by transwell migration assay. The migrated cells were photographed and counted (×200 magnification; scale bar, 100 μm). c Cell self-renewal capacity was measured by colony formation assay. d Cell apoptosis was detected by Annexin V-FITC assay and analyzed by FCM. e Cell growth inhibition rate of the two cells treated with 0–16 μg/ml cDDP for 24 h was analyzed using cell proliferation assay. f PP2A activity was determined by the Serine/Threonine Phosphatase Assay System. g-h The expression of related proteins B55δ, p-CDK1, CDK1, Cyclin B1, Cyclin E1, Bcl-2, Bax, and cleaved Caspase-3 was determined by WB assays. Data shown are the mean ± SD of three independent assays. *P < 0.01 as compared with Ctrl group of HepG2-shGFP cells; # P < 0.01 as compared with cDDP group of HepG2-shGFP cells