Fig. 6
CTNND1 regulates the transition between epithelial and mesenchymal phenotypes in HCC cells. a Representative phase-contrast images of SK-Hep-1 cells showed that CTNND1 silencing modulated morphologic changes. b Expression of epithelial (E-cadherin and α-catenin) and mesenchymal (N-cadherin and Vimentin) markers was analyzed by Western blotting in SK-Hep-1-shCTNND1 #1 cells and control cells. c Expression of epithelial (E-cadherin) and mesenchymal (N-cadherin) markers was analyzed by immunofluorescence stains in SK-Hep-1-shCTNND1 #1 cells and control cells. d Expression of epithelial and mesenchymal markers was analyzed by qRT-PCR in SK-Hep-1-shCTNND1 #1 cells and control cells. e Representative phase-contrast images of SNU-449 cells showed that CTNND1 overexpression modulated morphologic changes. f Expression of epithelial (E-cadherin and α-catenin) and mesenchymal (N-cadherin and Vimentin) markers was analyzed by Western blotting in SNU-449-CTNND1 cells and control cells. g Expression of epithelial (E-cadherin) and mesenchymal (N-cadherin) markers was analyzed by immunofluorescence stains in SNU-449-CTNND1 cells and control cells. h Expression of epithelial and mesenchymal markers was analyzed by qRT-PCR in SNU-449-CTNND1 cells and control cells. **, P < 0.01 is based on the Student t test. All results are from three independent experiments. Error bars, SD