Fig. 8

CTNND1 direct interacts with Kaiso and promotes WNT11, Cyclin D1, and MMP7 expression. a 293T cells were co-transfected with HA-tagged CTNND1 and Myc-tagged Kaiso constructs. Immunoprecipitation and subsequent immunoblot analysis revealed that HA-tagged CTNND1 co-immunoprecipitated with Myc-tagged Kaiso. b Expression of WNT11, Cyclin D1, and MMP7 protein was measured by Western blotting in 293T cells transfected with HA-tagged CTNND1 and control cells. c Expression of WNT11, Cyclin D1, and MMP7 protein was measured by Western blotting in SK-Hep-1-shCTNND1 #1 cells and control cells. d Expression of WNT11, Cyclin D1, and MMP7 mRNA was measured by qRT-PCR in SK-Hep-1-shCTNND1 #1 cells and control cells. e Expression of WNT11, Cyclin D1, and MMP7 protein was measured by Western blot in SNU-449-CTNND1 cells and control cells. f Expression of WNT11, Cyclin D1, and MMP7 mRNA was measured by qRT-PCR in SNU-449-CTNND1 cells and control cells. **, P < 0.01 is based on the Student t test. All results are from three independent experiments. Error bars, SD