Fig. 3From: Prohibitin overexpression predicts poor prognosis and promotes cell proliferation and invasion through ERK pathway activation in gallbladder cancerSilencing of PHB expression inhibited GBC cell proliferation in vitro. a The viability of NOZ and SGC-996 cells at the indicated time points after transfection was evaluated using a CCK-8 cell viability assay. b DNA synthesis in Lv-sh NC and Lv-sh PHB GBC cells was examined using the Edu incorporation assay. Representative fluorescence images are shown. c Cell cycle distribution in Lv-sh NC and Lv-sh PHB GBC cells was analyzed by flow cytometry. Representative fluorescence histograms and the percentage of cells in each phase are shown. (*p < 0.05 compared with the Lv-sh NC group)Back to article page