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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Prohibitin overexpression predicts poor prognosis and promotes cell proliferation and invasion through ERK pathway activation in gallbladder cancer

Fig. 5

Downregulation of PHB expression induced a dramatic reduction in the growth and metastasis of GBC cells in vivo. a Subcutaneously established NOZ cell-derived tumors in nude mice were monitored every 4 days until the mice were sacrificed on the 28th day. The harvested xenografts and tumor growth curves are presented. b and c The peritoneal invasion and tail vein-lung metastasis tumor models were employed to confirm the pro-metastatic role of PHB in GBCs. The peritoneal invasion and lung metastasis occurrence are shown, respectively. d PHB, p-ERK, PCNA and MMP-9 expression levels in harvested tumor tissues were determined using an IHC staining assay. e The extent of cell apoptosis in the tumor specimens was detected using a TUNEL apoptosis assay. Representative fluorescence images and the mean percentages of apoptosis cells are shown. (*p < 0.05 compared with the Lv-sh NC group)

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