Fig. 2
Effect of Zt/g4-DM1 on BC and NSCLC cell cycle, survival, and death. a Cell cycle changes: BC and NSCLC cell lines (1 × 106 cells per dish) were treated at 37 °C with 5 μg/ml of Zt/g4-DM1 for various times, collected, stained with propidium iodide, and then analyzed by flow cytometer as previously described [36]. MCF-7 cells were used as the control. Changes in cell cycle were marked with arrows. b Reduction of cell viability: BC and NSCLC cell lines (5000 cells per well in a 96-well plate in triplicate) were treated with different amounts of Zt/g4-DM1 for 96 h. Cell viability was determined by the MTS assay. The IC50 values were calculated using GraphPad Prism 6 software. c The kinetic effect of Zt/g4-DM1 on cell viability. H358 and H2228 cells were cultured and treated with Zt/g4-DM1 as described in a. Cells were collected at 24, 48, 72, and 96 h followed by the MTT assay