Fig. 3
Induction of cell apoptosis by Zt/g4-DM1. a morphological analysis of cell death. BC and NSCLC cells were treated with different amount of Zt/g4-DM1 for 96 h. Morphological changes were observed under the Olympus BK-41 inverted microscope and photographed. Images showing cell death are presented. b Dose-dependent cell death. BC and NSCLC cells were cultured and treated with Zt/g4-DM1 for 96 h as described in Fig. 1. The percentages of cell death from individual cell lines were determined by the trypan blue exclusion assay. The IC50 values of cell death were calculated using the GraphPad Prism 6 software. c PARK cleavage as cellular apoptosis. BC and NSCLC cells (2 × 106 cells in a 60 mm diameter dish) were treated with different amounts of Zt/g4-DM1 as described in Fig. 1. Cells were collected at different intervals. Cellular proteins (50 μg per samples) were subjected to Western blot analysis using an antibody specific to PARP fragment. Results shown here are from one of two experiments with similar results