Fig. 1

TET3 was downregulated in TGF-β1-treated ovarian cancer cells. SKOV3 and 3AO cells were maintained in 1640 medium containing 1 % newborn bovine serum with or without 10 ng/ml TGF-β1 for 48 h. a Quantitative real-time PCR showed that TET3 was significantly decreased at mRNA level in cells treated by TGF-β1, and TET1 was also downregulated in TGF-β1-treated SKOV3 cells. b Western blot results and (c) the quantitative analysis revealed that TET3 protein was decreased in cells stimulated by TGF-β1. All experiments were carried out in triplicate and the results were presented as means ± SD. *P < 0.05, **P < 0.01, t-test