Fig. 4

TET3 was an upstream regulator of miR-30d. a Quantitative real-time PCR showed that decreased miR-30s by TGF-β1 was reversed by restoration of TET3 both in SKOV3 and 3AO cells. b Western blot results and (c) the quantitative analysis indicated that resume of miR-30d could not reverse the downregulation of TET3 induced by TGF-β1 in ovarian cancer cells. d MSP assay proved that methylation of miR-30d precursor gene was increased in TGF-β1-treated cells. e Quantitative analysis of MSP results showed that methylated proportion of miR-30d precursor gene in FH-TET3-pEF transfected and TGF-β1 co-treated cells was lower than TGF-β1-treated cells. *P < 0.05, **P < 0.01, t-test