Fig. 7

In vitro and in vivo apoptotic findings. a Flow cytometric analysis of apoptosis induction in HCC cells-treated Ad-ΔB/TRAIL plus Ad-ΔB/IL-12 (Ad-ΔB/TRAIL+IL-12), in comparison with cells treated with control Ad-ΔB or PBS, by using Annexin-V and propidium iodide (PI) fluorescence staining assay. Each scatter plot demonstrates the percentage of early apoptotic cells (Annexin-V⁺cells, bottom right quadrant) and late apoptotic cells (PI⁺cells, upper right quadrant). b Western blot analysis showing the intra-tumor expression levels of cleaved caspase-3 (left panel) and caspase-8 (right panel) at day 3 after treatment of HCC-bearing mice with PBS, Ad-ΔB, or Ad-ΔB/TRAIL + Ad-ΔB/IL-12 (Ad-ΔB/TRAIL+IL-12) at a dosage regimen of 1 × 10¹⁰ VP in 200 μL PBS of each virus; repeated three times every other day