Fig. 3

SP600125 potentiated inhibitory effects of isoform-selective PI3K inhibitors on glioblastoma cell migration. a Number of viable U-87 MG cells was counted using Vi-Cell Cell Viability Analyzer (Beckman Coulter) before and after treatment of mitomycin C for 24 h. Cell proliferation was inhibited by mitomycin C at 5 or 10 μg/mL without impairing viability. b Wound healing in U-87 MG cells treated with PIK-75 (0.1 μM), TGX-221 (20 μM) or CAL-101 (10 μM) alone and combined with SP600125 (20 μM) for 24 h. Cells were pretreated with 5 μg/mL of mitomycin C for 1 h. The lines indicate the edge of wound generated before drug treatment (0 h). Photographs were obtained at 50× magnification. c-e Migration rate was analyzed and expressed as the number of cells migrating into the original wounds. U-87 MG cell migration was blocked by PIK-75, TGX-221, CAL-101 and SP600125 alone. Inhibition of migration rate was reinforced by the combined treatment of TGX-221 and SP600125, as well as CAL-101 and SP600125 (n = 3; p values were determined by One-way ANOVA and Post Hoc multiple comparison Tukey HSD test. *: p <0.05; **: p <0.01; ***: p <0.001)