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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: PI3K p110β isoform synergizes with JNK in the regulation of glioblastoma cell proliferation and migration through Akt and FAK inhibition

Fig. 5

SP600125 potentiated inhibitory effects of isoform-selective PI3K inhibitors on lamellipodia and membrane ruffles formation. a Immunocytochemistry of U-87 MG cells stained with Alexa Fluor 594-conjugated phalloidin (red) after treatment with PIK-75 (0.1 μM), TGX-221 (20 μM) or CAL-101 (10 μM) alone and combined with SP600125 (20 μM) for 3 h. Lamellipodia (white arrow) were decreased after inhibition of p110α, p110β, p110δ and JNK, while stress fibers (arrowhead) were widely developed at the presence of SP600125. Bar = 50 μm. b-d Cells with lamellipodia were counted and data were expressed as the ratio of number of cells with lamellipodia over total cell number. SP600125 potentiated the inhibitory effect of TGX-221 and CAL-101 on lamellipodia formation (n = 3; p values were determined by One-way ANOVA and Post Hoc multiple comparison Tukey HSD test. ***: p <0.001). e Phase contrast microscopy of U-87 MG cells after treatment with PIK-75, TGX-221 or CAL-101 alone and combined with SP600125 for 3 h. Cells with membrane ruffles (black arrow) were reduced by the treatment of isoform-selective PI3K inhibitors and SP600125. Photographs were obtained at 200× magnification. f-h Cells with membrane ruffles were counted and data were expressed as the ratio of number of cells with membrane ruffles over total cell number. SP600125 also enhanced the inhibitory effect of TGX-221 and CAL-101 on membrane ruffles formation (n = 3; p values were determined by One-way ANOVA and Post Hoc multiple comparison Tukey HSD test. **: p <0.01; ***: p <0.001)

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