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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: PI3K p110β isoform synergizes with JNK in the regulation of glioblastoma cell proliferation and migration through Akt and FAK inhibition

Fig. 6

Isoform-selective PI3K inhibitors and SP600125 affected focal adhesion and cytoskeleton-related signaling. a-c U-87 MG cells were treated with PIK-75 (0.1 μM), TGX-221 (20 μM) or CAL-101 (10 μM) alone and combined with SP600125 (20 μM) for 24 h. Whole cell lysates were separated by SDA-PAGE, and the phosphorylated and total protein levels were determined by Western blotting using antibodies against the corresponding phosphorylated and total proteins. Expression of GAPDH was served as a loading control. Data were representative of three independent experiments. Density of FAK d and zyxin e phosphorylation levels were relative to total FAK and zyxin protein expression levels. Combination of TGX-221 and SP600125 showed a higher inhibition of FAK phosphorylation, while combination of CAL-101 and SP600125 displayed higher inhibition of FAK and zyxin phosphorylation (n = 3; p values were determined by One-way ANOVA and Post Hoc multiple comparison Tukey HSD test. *: p <0.05; **: p <0.01)

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