Fig. 7
Combined inhibition of p110β and JNK exerted synergism to suppress xenograft tumor growth in vivo. Human glioblastoma U-87 MG cells (5 × 106 cells) were subcutaneously injected into the Balb/C nude mice. Nine days after inoculation, mice were intraperitoneally (i.p.) injected once daily for 7 days with vehicle, TGX-221 (40 mg/kg) and CAL-101 (20 mg/kg) alone, or in combination with SP600125 (40 mg/kg). Measurement of tumor volumes started on the day of the first administration. a, c Representative subcutaneous tumor xenografts from mice sacrificed after 37-day post-administration. b, d Summary data of tumor volumes from 10-day post-administration to the end of the experiment. (n = 6; p values were determined by One-way repeated measures ANOVA and Post Hoc multiple comparison Tukey HSD test. *: p <0.05; ***: p <0.001 compared with vehicle control; a: p <0.05 compared with PI3K inhibitor; b: p <0.05 compared with SP600125)