Fig. 5

SH2B1 is a direct downstream target of miR-361-3p. a Schematic of the construction of wild-type or mutant SH2B1 3'-UTR vectors is illustrated. b Relative luciferase activity was analyzed in A549 and HTB-182 cells. Firefly luciferase vector was used as an internal control. c Western blot results of SH2B1 protein in A549, HTB-182 and SPC-A-1 cells infected with miR-361-precursor lentivirus or anti-miR-361-p. d The representative IHC pictures showed the SH2B1 protein expression in NSCLC tissue with high or low miR-361-3p expression. e The inverse correlation between the expression of miR-361-3p and SH2B1 in the same set of NSCLC tissue specimens. Data were represented as the mean ± SEM of three independent experiments. **P < 0.01