Fig. 3
From: FLIPL is critical for aerobic glycolysis in hepatocellular carcinoma
FLIPL inhibits the ubiquitination and degradation of SGLT1 by interacting with SGLT1. a The localization of FLIPL and SGLT1 were determined in HepG2 cells by laser confocal scanning microscopy. Green fluorescence indicated FLIPL expression, red fluorescence indicated SGLT1 expression, and blue fluorescence indicated nuclear staining with DAPI. Bar = 20 μm. b HepG2 cells were harvested for co-immunoprecipitation assays followed by immunoblot (IB) analysis. Immunoprecipitation (IP) assays were performed with whole-cell lysates (WCE) pretreated with A/G–conjugated Sepharose beads. WCE were probed for input. The antibodies used for IP and IB are as indicated. c SGLT1 expression was determined in HepG2 cells with FLIPL overexpression for 48 h and/or 5 μM MG132 for another 4 h. d HEK293 cells were transfected with hemagglutinin (HA)-ubiquitin plasmid, Flag-FLIPL plasmid, and Myc-SGLT1 plasmid for 48 h. Subsequently, the cell lysates were collected and analyzed by IP and IB. e HepG2 cells were transfected with HA-ubiquitin and shFLIPL plasmids for 48 h, and cell lysates were collected and analyzed by IP and IB