Fig. 2

Example of a represented binary percentages of positive staining (frequency of protein expression) in breast cancer. Staining was categorized as high, medium, low or not detected for each antibody by the Human Protein Atlas (proteinatlas.org). For the purposes of applying a binary system of enumeration, only high or medium scores were considered positive staining. The number of these positive stains was divided by the number of total stains performed to ascertain the percentage of positive staining. For example, for YY1 antibody HPA001119, there were 2 high and 5 medium scores. That comprises 7 out of a total 9 stains for a total of 78 % positive staining. The graph compares these percentages of positive staining for each antibody—YY1 antibody HPA001119, YY1 antibody CAB009392, SOX2 antibody CAB010648, NANOG antibody CAB019380, BMI1 antibody HPA030472, BMI1 antibody CAB011120, POU5F1 antibody CAB025600 and POU5F1 antibody CAB026380—in breast cancer