Transfer of malignant trait to BRCA1 deficient human fibroblasts following exposure to serum of cancer patients

Table 2 Primers sequences used in sgBRCA1 cloning and knockout validation

Primer	Sequence (5’-3’)	Purpose
sgBRCA1^a	CACCGTGGTCACACTTTGTGGAGAC AAACGTCTCCACAAAGTGTGACCAC	Single-guide cloning
hU6_Seq	ACTATCATATGCTTACCGTAAC	Primer for sequencing
BRCA1-F	TAGGGGTGGATATGGGTGAA	Surveyor assay
BRCA1-R	GTTGCAGTGAGCCAAGATCA	Surveyor assay

^aThese oligos were annealed to make the double-stranded linker with sticky ends (underlined nucleotides)

ISSN: 1756-9966