Fig. 5

shRNA-mediated downregulation of RNF8 inhibits tumor metastasis in vivo. a Bioluminescence images in vivo. A total of 1 × 10⁶ /0.1 mL MDA-MB-231-luc cells stably expressing shRNF8-2 or an empty vector control were injected into caudal vein of 6–8 week-old female BALB/c mice (n = 6) (left), lung metastases were quantified using bioluminescence imaging. Lungs were harvested via necropsy 43 days after caudal vein injection and were examined by in vitro bioluminescent measurement (right). b Representative images of hematoxylin and eosin staining of lung sections. (magnification, 4 ×, left; 40 ×, right). Metastatic nodules were indicated by green arrows. c The number of mice with metastatic nodules in the lung sections. d Before injection, the expression of RNF8 and E-Cadherin in MDA-MB-231-luc-siCon and MDA-MB-231-luc-shRNF8-2 cells were examined by Western blotting