Fig. 6

RNF8 is frequently upregulated in breast bancer tissues. a Expression of RNF8 was examined by IHC in primary breast cancers (Malignant) and the paired adjacent normal tissues (NAT). b RNF8 is differentially expressed in primary malignant breast cancer tissue compared with the corresponding paired adjacent normal tissue. For each case of the 36 patients, index of RNF8 expression (immunostaining score of IHC) of the primary tissue was subtracted by that of the corresponding paired adjacent normal tissue, and the resultant values (Malignant-NAT) from all 36 cases were used for plotting. The relative RNF8 expression level between primary cancer and paired adjacent tissue of each case was represented as a dot. RNF8 is considered to be significantly upregulated, similar or negative in primary tissue when compared with the corresponding paired adjacent tissue only if the calculated index is >0 (red dots), 0 (green dot), <0 (blue dots), respectively