Fig. 3
Cell migration and invasion assays: The experiments were performed in transwell chambers with non-Matrigel or Matrigel coated membranes by using stable transfected SK-Hep-1 cell lines with pCMV-Tag2A-wX, pCMV-Tag2A-X and control vectors. Cells were stained with crystal violet and photographed (magnification, ×200). Data shown as mean ± SD of each group from three independent experiments. Compared with the control cells, HBx cells showed significantly enhanced migration and invasion capacity (**P < 0.01, *P < 0.05), HBwx also showed a promoting effect on migration and invasion. However, the migratory and invasive ability of HBwx cells was significantly reduced compared with HBx cells (**P < 0.01, *P < 0.05)