Fig. 6

miR-200a directly targeted the 3′UTR of oncogene TGF-β2 and inhibits its expression in glioma cells. a The predicted of miR-200a binding sites in the 3′-UTR region of TGF-β2 (TGF-β2-3′-UTR-WT) and the corresponding mutant sequence (TGF-β2-3′UTR-MUT) was shown. b-c Relative expression of TGF-β2 mRNA and protein levels in U251 and A172 cells after transfected with miR-200a mimics, miR-200a inhibitors, and miR-200a NC. *P < 0.05, **P < 0.01 vs. miR-200a NC group. d-e Luciferase activity in U251 and A172 cells co-transfected with miR-200a mimics and luciferase reporters containing TGF-β2 wild type (WT) or mutant type (MUT) 3′-UTR. **P < 0.01 vs. miR-200a NC group. Data are presented as mean ± SD from three independent experiments