Fig. 5

ADAM10 is a direct target of miR-655-3p in HCC. a. qRT-PCR analysis of ADAM10 mRNA expression after the miR-655-3p agomiR, or antagomiR transfection in HCC cells. b, d. Western blot analysis of ADAM10 protein expression after the miR-655-3p agomiR, or antagomiR transfection in HCC cells; and their Column charts analysis. c. The predicted interaction site of miR-655-3p and candidate target gene ADAM10 wild-type 3’-UTR and serial deleted forms of the 3’UTR reporters. e, f. Luciferase assay of co-transfected with miR-655-3p agomiR and pMIR-REPORT-ADAM10 plasmid (miR-NC and miR-655-3p with ADAM10 WT 3’UTR; miR-NC and miR-655-3p with ADAM10 MUT 3’UTR) after 24 h. Data are mean ± SEM (n = 3). *p < 0.05, and **p < 0.01 compared to controls