Fig. 1
MUC4/Y and MUC4/Y-AMOP domain-deletion constructs and their stable expression in PANC-1 cells. a The structure diagram of the MUC4, MUC4/Y and MUC4/Y-AMOP△. Different domains present in MUC4 protein are the central large tandem repeat domain, NIDO, AMOP, vWD, and 3 carboxyl-terminal located EGF domains. b MUC4/Y expression was analysed by real-time PCR in different PANC-1 derived sub-lines. GAPDH was used as an internal control. c Western blotting was conducted to analyse the protein level of MUC4/Y in different groups. GAPDH was probed as an internal control. d Cell confocal immunofluorescence assay showed that the MUC4/Y and the truncated protein (MUC4/Y-AMOPΔ) could anchor on the cytomembrane and cytoplasm.**P < 0.01