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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: The role of the AMOP domain in MUC4/Y-promoted tumour angiogenesis and metastasis in pancreatic cancer

Fig. 3

Matrigel plug assay and the correlation between MUC4/Y-AMOP domain and mouse survival. a Cells were suspended in Matrigel and DMEM and subcutaneously into the flanks of mice (n = 8). After 15 days, the Matrigel plugs were removed and photographed. Representative Matrigel plugs from the PANC-EV, PANC-MUC4/Y, and PANC-MUC4/Y-AMOPgroups are shown. b The relative amount of angiogenesis was analysed based on the RBC haemoglobin level, determined using the Drabkin method. The relative haemoglobin content is the haemoglobin level (mg) divided by the final volume of each plug. c CD31 immunohistochemistry was performed on the Matrigel plugs from the PANC-EV, PANC-MUC4/Y, and PANC-MUC4/Y-AMOPgroups. d Microvessel density of CD31 immunohistochemistry. Each slide was evaluated with five fields, and the data were analysed as the mean vessel number of these five fields. e Kaplan-Meier survival curves in 45 mice with PANC-1 derived sub-lines orthotopically implanted. The p values were calculated by the log-rank test (Table 1)

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