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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: The role of the AMOP domain in MUC4/Y-promoted tumour angiogenesis and metastasis in pancreatic cancer

Fig. 5

Mechanisms involved in MUC4/Y-AMOP domain regulating angiogenesis and metastasis. a Effects of MUC4/Y and its AMOP domain on the NOTCH3 signalling pathway (NOTCH3, N3ICD, HES-1) were assayed by western blotting. GAPDH was used as the internal control. b Immunofluorescence was used to assess the NOTCH3 and MUC4(MUC/Y) protein level. c Western blotting assays were conducted to analyse the protein level of VEGF-A, MMP-9, ANG-2 and N3ICD in different groups. GAPDH was used as the internal control. d The secreted level of VEGF-A in conditioned medium was detected by an ELISA kit assay. e The activity of MMP-9 in different groups was detected by using a Fluorokine E Human MMP-9 Activity Assay kit assay. f The mRNA expression of ANG-2 in different groups. DAPT: γ-secretase inhibitors (GSI) can inhibit the proteolytic processing of Notch receptors by γ-secretase, which is essential for Notch activation. **P < 0.01

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