Fig. 4

The E545K mutation of PIK3CA increased downstream Akt activity. a-g The functional proteins associated with the PI3K-akt pathway in cancer progression were examined by Western blot assays. Representative chemiluminescent images and the relative expression quantities of cyclin D1, bcl-2, Bax, vimentin and E-cadherin (mean ± SD, n = 3) are shown. h, i Key proteins in the PI3K-akt pathway were examined by Western blot assays. Representative chemiluminescent images of PI3K p110α, p-akt and akt and the relative expression quantities of p-akt (mean ± SD, n = 3) are shown