Fig. 5

The E545K mutation enhanced PI3K p110α binding to EGFR. a, b After cellular starvation, EGF was added to the GBC-SD and NOZ cells. EGFR and key proteins in the PI3K-akt pathway were examined by Western blot assays. Representative chemiluminescent images of PI3K p110α, p-akt, akt and EGFR and the relative expression quantities of p-akt (mean ± SD, n = 3) are shown. c, d PI3K p110α binding to EGFR was examined by co-immunoprecipitation assays. Representative chemiluminescent images and the relative expression quantities of PI3K p110α binding to EGFR (mean ± SD, n = 3) are shown