Fig. 7

The E545K mutation promoted GBC progression and the selective p110α inhibitor suppressed GBC progression in vivo. a Mice bearing subcutaneous xenografts of CON, WT and E545K cells were anesthetized and photographed after three weeks. b The tumor volumes of CON, WT and E545K cell subcutaneous xenografts were measured every three days after inoculation. Data are presented as mean ± SD (n = 5). c Mice bearing WT and E545K cell subcutaneous xenografts were injected with 1 mg A66 or 100 μl NS every 3 days after inoculation for three weeks. Tumor volumes were measured every three days. Data are presented as mean ± SD (n = 5). d Representative photomicrographs of immunohistochemical staining for tumors resected from mice treated with A66 or NS are shown. e Percent of Ki67 positive cells is shown