Fig. 3

Knockdown of HOXD-AS1 by siRNA or tet-shRNA inhibits cell proliferation. CCK-8 assay was performed to investigate the cell growth curve. a, b Cell proliferation inhibition was detected in 5637 (P < 0.001) and T24 cells (P < 0.001) after transfection with si-HOXD-AS1 (c, d) The tet–shRNA induced by 1ug/ml dox significantly inhibits cell proliferation in 5637 (P < 0.001) and T24 cells (P < 0.001). Data were expressed as means ± SD from three independent experiments