Fig. 4

The multiplication capacity of 5637 and T24 cells was declined after transfection with si-HOXD-AS1 or tet-shRNA. EdU assay was performed. a, b The number of EdU positive cells was decreased significantly after transfected with si-HOXD-AS1 in 5637 and T24 cells. c The percentage of EDU positive cells in 5637 (P = 0.001) and T24 cells (P = 0.001) can be seen. d, e The number of EdU positive cells was decreased significantly after transfection with tet-shRNA which was induced by 1ug/ml dox. f, g The percentage of EdU positive cells in 5637 (P = 0.002) and T24 cells (P = 0.002) can be seen. Data were expressed as means ± SD from three independent experiments