Fig. 5

Vital cell count (a), Apoptosis (b), and Autophagy (c). Vital cell count was determined by trypan blue exclusion assay, apoptosis by FAM-FLICA™ in vitro Caspase 3/7 Detection Kit, and autophagy by Cyto-ID® Autophagy Detection Kit, 18 h after treatment with the single drugs (5-aza-dC, RES, ABC; concentrations see Table 1) or irradiation with 2 Gy. Data were normalized to the untreated control group and presented as mean ± SEM, n = 3. Statistical significances are presented vs. control by asterisks (*, p ≤ 0.05; **, p ≤ 0.01). The joint analysis included the relative data of the three MB cell lines