Fig. 7

Detection of DNA double-strand breaks (DSB). The DSB repair protein gH2AX was quantified by immune fluorescence microscopy after 1 h and 24 h treatment with the single drugs (5-aza-dC, RES, ABC; concentrations see Table 1) or 1 h and 24 h after irradiation with 2 Gy. a Representative images of gH2AX (red) and DAPI (blue) staining in DAOY cells; scale bar = 12 μm. b Treatment-induced gH2AX-foci relative to control cells in the single MB cell lines. At least 50 nuclei were counted with a maximum of 30 foci per cell. Experiment was performed once and data are presented as mean ± SEM. Statistical significances are presented vs. control by asterisks (*, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001)