Enhanced inhibition of clonogenic survival of human medulloblastoma cells by multimodal treatment with ionizing irradiation, epigenetic modifiers, and differentiation-inducing drugs

Table 1 Drug concentrations for the combinatorial treatment of human MB cells

cell line	D283-Med	MEB-Med8a	DAOY
5-aza-dC	0.05 μM	0.1 μM	0.5 μM
VPA	3 mM	1 mM	2 mM
SAHA	1 μM	2 μM	2 μM
Abacavir	0.25 mM	0.04 mM	0.24 mM
ATRA	1.5 μM	10 μM	10 μM
Resveratrol	11 μM	9.5 μM	40 μM

For combination experiments, IC 90 values were calculated from single drug clonogenic assays for each cell line separately and indicate the drug concentration leading to 90 % inhibition of colony formation. For SAHA and ATRA, the maximal tolerated plasma concentrations were used in MEB-Med8a and DAOY, leading to IC 71/IC 82 (SAHA) or IC 46/IC 72 (ATRA), respectively

ISSN: 1756-9966