Fig. 2

Silencing of SALL4 inhibits cell proliferation, induces apoptosis and arrests cell cycle in vitro. a Real-time PCR analysis of SALL4 expression in Het1A, TE1, TE7, EC1, EC109, EC9706, KYSE70, KYSE450 cell lines. b The mRNA level of SALL4 was verified in sorted TE7 and EC109 cells after transfection. c The protein level of SALL4 in sorted TE7 and EC109 cells was assessed by using Western blotting. β-actin was used as an internal control. d Cell viability was evaluated at indicated time points using CCK8 assay. e Cell apoptosis was measured by flow cytometric analysis. f Knock-down of SALL4 induced cell cycle arrest at G0/G1 phase. (*P < 0.05, **P < 0.01, ***P < 0.001)