Fig. 1

Synaptojanin 2 Binding Protein (SYNJ2BP) is frequently down-regulated in hepatocellular carcinoma(HCC) tissues and HCC cell lines. a The SYNJ2BP mRNA expression levels in 28 paired HCC tissues and peritumoral tissues (PTs) were obtained from quantitative real-time polymerase chain reaction (qRT-PCR). Fold inductions were calculated using the formula 2-(△△Ct), GAPDH was used as internal control. The expression of SYNJ2BP mRNA in PTs was used to normalize (value set to 1) the expression of SYNJ2BP mRNA in HCC tissues. b Western blot was also adopted to validate SYNJ2BP expression levels in HCC tissues and PTs. The protein expression level of SYNJ2BP in HCC tissues was significantly lower than that in PTs. *P < 0.05. But there were not significant differences between PTs and NLs (P = 0.442). c, d mRNA and protein expression levels of SYNJ2BP were detected by qRT-PCR and western blot in one normal liver cell line (L02) and 5 HCC cell lines. The results showed that compared to L02 cells, Hep3B cells had the highest SYNJ2BP expression level, while HCCLM3 cells had the lowest expression level. Data were normalized to the expression level of SYNJ2BP in L02. *P < 0.05. β-actin was used as the loading control