Fig. 6

Synaptojanin 2 Binding Protein (SYNJ2BP) stimulates Notch signaling in hepatocellular carcinoma (HCC). Previous HCC cells transfected with DLL4 expression or shDLL4 or control vectors were used in these studies. a Notch signaling was stimulated in cells overexpressing SYNJ2BP while the other signaling pathways showed no significant change. b Western blot was employed to detect expression levels of DLL4, DLL1 and VEGF, only DLL4 was positively related to SYNJ2BP. c Representative images showed that overexpression or silencing of DLL4 mimics or abrogates SYNJ2BP mediated cellular morphology changes of HCC cells. Data were normalized to the expression level of SYNJ2BP in untreated HCC cell. Cell nuclei were stained with 4, 6-diamidino-2-phenylindole (blue). Original magnification: 400× for C. *P < 0.05