Fig. 2
The expression of Nestin and the different chemo-sensitivities in various HCC cell lines. a The IC50 values of the parental (Bel-7402) and chemo-resistant cell lines (Bel-7402/5-FU and Bel-7402/ADM) were determined by MTT assays. b In all nine examined HCC cells lines, quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blotting demonstrated the high Nestin mRNA and protein expression in two highly invasive HCC cell lines (MHCC97H and HCCLM3) and in the two drug-resistant sublines (Bel-7402/5-FU and Bel-7402/ADM)