Fig. 3

Chemoresistant HCC sublines exhibit characteristics of epithelial-mesenchymal transition (EMT). a Cellular morphology of Bel-7402/5-FU and Bel-7402/ADM was significantly changed compared with Bel-7402. The wound-healing assay (b) and transwell assay (c) were performed to analyze the difference in motility and invasion between parental (Bel-7402) and drug-resistant cell lines (Bel-7402/5-FU and Bel-7402/ADM). The percentage of wound healing for cells passing through the transwell membranes of each well was calculated and is compared in the diagrams. d Western blotting assays showed the expression of EMT-related proteins in parental (Bel-7402) and drug-resistant cell lines (Bel-7402/5-FU and Bel-7402/ADM). e Immunofluorescence staining of nuclei, E-Cadherin and Vimentin. Images were taken using a fluorescense microscope under excitation at 405 nm, 488 nm, or 546 nm (Scale bars: 100 μm)