Fig. 4
The signaling pathways involved in Nestin-regulated HCC multidrug resistance (MDR). a The cell morphology of parental (Bel-7402) and resistant cell lines (Bel-7402/5-FU and Bel-7402/ADM) transfected with si-Nestin was significantly changed by 5-FU, L-OHP and ADM treatment. b MTT assay demonstrated significantly decreased cell viability in Si-Nestin-transfected resistant cell lines (Bel-7402/5-FU and Bel-7402/ADM) compared to Si-Controls, following 5-FU, L-OHP and ADM treatments respectively (all P < 0.05). c The signaling pathways involved in Nestin-regulated MDR of HCC cells. As compared to the Bel-7402-control, levels of GSK-3β and p-β-Catenin were significantly lower, while p-GSK-3βSer9, total β-catenin, survivin, C-myc, Bcl-2 and nuclear β-catenin levels were higher in Bel-7402-Nestin