Experimental metastasis (Fig. 1)
|
1-MNA
|
Per os in drinking water
|
100 mg/kg/day
|
Treatment initiated 7 days prior cancer transplantation and continued to the end of the experiment
|
1,4-DMP
|
Spontaneous metastasis (single drug treatment) (Fig. 2)
|
1-MNA
|
Per os in drinking water
|
100 mg/kg/day
|
Continuously from the 7th day of the experiment to the end of the experiment
|
1,4-DMP
|
Matrigel plugs (Fig. 3)
|
1-MNA
|
Per os in drinking water
|
100 mg/kg/day
|
Continuously starting from the day of matrigel plugs transplantation
|
1,4-DMP
|
Cyclophosphamide
|
intraperitoneally
|
25 mg/kg/day
|
On days 0, 3, 5
|
Spontaneous metastasis (combined treatment) (Fig. 4 and 5)
|
1-MNA
|
Per os in drinking water
|
100 mg/kg/day
|
Continuously from the 1st day of the experiment to the end of the experiment
|
1,4-DMP
|
Cyclophosphamide
|
Intraperitoneally
|
25 mg/kg/day
|
From the 7th day of the experiment, 3 times each week
|