Fig. 3
From: CD317 Promotes the survival of cancer cells through apoptosis-inducing factor
CD317 knockdown promotes cell apoptosis through caspase-independent manner. a Blot image represented 1 of 3 independent experiments performed in Hela cells. NC and siR317 cells were cultured in the presence or absence of FBS and harvested at 24 h. Whole cell lysates were prepared and analyzed for LC3, Caspase 8, 9, 3 and CD317. β-actin served as an equal loading control. b Representative graphs of cell apoptosis determined by flow cytometric evaluation in Hela cells. NC and siR317 cells were cultured in FBS-free medium with or without 40 μM Ac-DEVD-CHO (a specific inhibitor for Caspase 3) and harvested at 48 h for apoptosis analysis. c Quantitative analysis of cell apoptosis as shown in B. *P < 0.05