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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: CD317 Promotes the survival of cancer cells through apoptosis-inducing factor

Fig. 5

CD317 overexpression inhibits serum deprivation-induced apoptosis in AIF-related manner. a. Representative graphs (left panel) and statistical analysis (Right panel) of cell apoptosis determined by flow cytometric evaluation in HEK293T cells. In brief, 36 h post transfection,cells were cultured in the indicated conditions for another 48 h and stained with 7-AAD to detect cell apoptosis. **P < 0.005. b. Subcellular distribution of AIF in HEK293T cells was detected by western blot. In brief, MigR1 and plasCD317 cells were cultured in the presence or absence of FBS for 24 h and collected for total protein or subcellular fractionation extraction. Lamin A/C, β-Actin, and COX IV served as the loading control for nuclear, total, and mitochondrial protein, respectively

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