Fig. 5

Effects of different routes of CDDP administration on remaining viable tumor tissue. a Number of cleaved PARP-1⁺ tumor cells. PARP-1⁺ tumor cells were only counted within areas of viable tumor tissue. b Representative images of tumor tissue (i.v. CDDP, i.a. CDDP) stained for HIF-1α (20X). Scale bar corresponds to 100 μm. c Quantitation of HIF-1α⁺ tumor tissue normalized to the entire viable tumor tissue available for evaluation. d Representative images of healthy adjacent tissue (muscle) and tumor tissue (i.v. CDDP, i.a. CDDP) stained for CD31 (20X), where the upper image shows CD31 expression in the endothelial cells lining capillaries as well as the larger vessels in the skeletal muscle surrounding the tumors, the central image represents CD31 expression after i.v. CDDP and the lower image displays an increase in CD31 expressing cells within the tumor mass after i.a. CDDP. Scale bar corresponds to 100 μm. e Quantitation of CD31⁺ tumor tissue normalized to the entire tumor tissue available for evaluation. f Correlation of HIF-1α IHC with CD31 IHC (Pearson’s r = 0.62). *p < 0.05; **p < 0.01 as compared to the indicated treatment