Fig. 5

JNK-c-Jun signal pathway may contributes to radiation-enhanced FasL expression. a SMMC7721 cells were treated with chemical inhibitors, SB203580 (p38), PD98059 (MAPK/ERK), LY294002 (PI3K/Akt), and SP600125 (SAPK/JNK) respectively for 60 min before irradiation. FasL and phosphorylated forms of p38, Akt, ERK, JNK in HCC cells were analyzed after be irradiated with 4Gy exposure and incubated for 24 h. b Activation of the JNK-c-Jun signaling pathway was investigated by Western Blot. c The protein levels of FasL in culture supernatant were determined using ELISA. d FasL expression after c-Jun silencing. *P < 0.05