Activation of the JNK-c-Jun pathway in response to irradiation facilitates Fas ligand secretion in hepatoma cells and increases hepatocyte injury

Table 1 Primer pairs used for qRT-PCR

Gene symbol	Sequence 5′-3′
FasL	Forward: 5′-TGCCTTGGTAGGATTGGGC- 3′
FasL	Reverse: 5′-GCTGGTAGACTCTCGGAGTTC - 3′
TRAIL	Forward: 5′-TGCGTGCTGATCGTGATCTTC- 3′
TRAIL	Reverse: 5′-GCTCGTTGGTAAAGTACACGTA- 3′
TNF-α	Forward: 5′-CCCGACTATCTCGACTTTGC- 3′
TNF-α	Reverse: 5′-GGTTGAGGGTGTCTGAAGGA- 3′
IL-6	Forward: 5′-CAATGAGGAGACTTGCCTGG- 3′
IL-6	Reverse: 5′-GGCATTTGTGGTTGGGTCAG - 3′
IGF-1	Forward: 5′-GCTCTTCAGTTCGTGTGTGGA- 3′
IGF-1	Reverse: 5′-GCCTCCTTAGATCACAGCTCC - 3′
PDGFA	Forward: 5′-GCAAGACCAGGACGGTCATTT- 3′
PDGFA	Reverse: 5′-GGCACTTGACACTGCTCGT- 3′
PDGFB	Forward: 5′-CTCGATCCGCTCCTTTGATGA- 3′
PDGFB	Reverse: 5′-CGTTGGTGCGGTCTATGAG - 3′
VEGFA	Forward: 5′-AGGGCAGAATCATCACGAAGT- 3′
VEGFA	Reverse: 5′-AGGGTCTCGATTGGATGGCA - 3′
TGF-β1	Forward: 5′-AGGACTGCGGATCTCTGTGT - 3′
TGF-β1	Reverse: 5′-GGGCAAAGGAATAGTGCAGA - 3′
GAPDH	Forward: 5′- CTCCTCCACCTTTGACGC - 3′
GAPDH	Reverse: 5′- CCACCACCCTGTTGCTGT- 3′

qRT-PCR quantitative real time reverse transcription polymerase chain reaction

ISSN: 1756-9966