Fig. 2

Inhibitory effect of RES on IL-17 production. PANEL a show the inhibitory effect of different concentrations of RES on IL-17 production. MT-2 cells and HTLV-1 infected CD4+ T cell cultures were treated with RES at 0.612,1.25,2.5,5,10,20 and 40 μg/ml for 48 h of culture. IL-17 production was assayed by ELISA and the results are expressed as IC50, i.e., the concentration of RES where the IL-17 production is reduced by 50 %. The bars represent the confidence limits. PANEL b show the Effect of RES on IL-17 transcription in MT-2 cells and HTLV-1 infected CD4+ cells. Cells were treated with RES at 20 μg/ml for 48 h and IL-17 transcription was assessed by RT-PCR. The image refers to visualization of IL-17 mRNA expression in DMSO treated MT-2 cells, RES-treated MT-2 cells, DMSO treated HTLV-1 infected CD4+ cells and RES-treated HTLV-1 infected CD4+ cells